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dc.contributor.authorPettitt, SJen_US
dc.contributor.authorKrastev, DBen_US
dc.contributor.authorPemberton, HNen_US
dc.contributor.authorFontebasso, Yen_US
dc.contributor.authorFrankum, Jen_US
dc.contributor.authorRehman, FLen_US
dc.contributor.authorBrough, Ren_US
dc.contributor.authorSong, Fen_US
dc.contributor.authorBajrami, Ien_US
dc.contributor.authorRafiq, Ren_US
dc.contributor.authorWallberg, Fen_US
dc.contributor.authorKozarewa, Ien_US
dc.contributor.authorFenwick, Ken_US
dc.contributor.authorArmisen-Garrido, Jen_US
dc.contributor.authorSwain, Aen_US
dc.contributor.authorGulati, Aen_US
dc.contributor.authorCampbell, Jen_US
dc.contributor.authorAshworth, Aen_US
dc.contributor.authorLord, CJen_US
dc.coverage.spatialEnglanden_US
dc.date.accessioned2017-03-24T12:54:35Z
dc.date.issued2017-03-01en_US
dc.identifierhttps://www.ncbi.nlm.nih.gov/pubmed/28248920en_US
dc.identifiersdata201720en_US
dc.identifier.citationSci Data, 2017, 4 pp. 170020 - ?en_US
dc.identifier.urihttps://repository.icr.ac.uk/handle/internal/484
dc.identifier.eissn2052-4463en_US
dc.identifier.doi10.1038/sdata.2017.20en_US
dc.description.abstractWe describe a screen for cellular response to drugs that makes use of haploid embryonic stem cells. We generated ten libraries of mutants with piggyBac gene trap transposon integrations, totalling approximately 100,000 mutant clones. Random barcode sequences were inserted into the transposon vector to allow the number of cells bearing each insertion to be measured by amplifying and sequencing the barcodes. These barcodes were associated with their integration sites by inverse PCR. We exposed these libraries to commonly used cancer drugs and profiled changes in barcode abundance by Ion Torrent sequencing in order to identify mutations that conferred sensitivity. Drugs tested included conventional chemotherapeutics as well as targeted inhibitors of topoisomerases, poly(ADP-ribose) polymerase (PARP), Hsp90 and WEE1.en_US
dc.format.extent170020 - ?en_US
dc.languageengen_US
dc.language.isoengen_US
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/en_US
dc.subjectAnimalsen_US
dc.subjectAntineoplastic Agentsen_US
dc.subjectDNA Transposable Elementsen_US
dc.subjectGenome-Wide Association Studyen_US
dc.subjectHaploidyen_US
dc.subjectMiceen_US
dc.subjectMouse Embryonic Stem Cellsen_US
dc.subjectNeoplasmsen_US
dc.titleGenome-wide barcoded transposon screen for cancer drug sensitivity in haploid mouse embryonic stem cells.en_US
dc.typeJournal Article
dcterms.dateAccepted2017-01-05en_US
rioxxterms.versionofrecord10.1038/sdata.2017.20en_US
rioxxterms.licenseref.startdate2017-03-01en_US
rioxxterms.typeJournal Article/Reviewen_US
dc.relation.isPartOfSci Dataen_US
pubs.notesNo embargoen_US
pubs.organisational-group/ICR
pubs.organisational-group/ICR/Primary Group
pubs.organisational-group/ICR/Primary Group/ICR Divisions
pubs.organisational-group/ICR/Primary Group/ICR Divisions/Breast Cancer Research
pubs.organisational-group/ICR/Primary Group/ICR Divisions/Breast Cancer Research/Gene Function
pubs.organisational-group/ICR/Primary Group/ICR Divisions/Cancer Biology
pubs.organisational-group/ICR/Primary Group/ICR Divisions/Cancer Biology/Development & Cancer
pubs.organisational-group/ICR/Primary Group/ICR Divisions/Molecular Pathology
pubs.organisational-group/ICR/Primary Group/ICR Divisions/Molecular Pathology/Development & Cancer
pubs.organisational-group/ICR/Primary Group/ICR Divisions/Molecular Pathology/Gene Function
pubs.publication-statusPublished onlineen_US
pubs.volume4en_US
pubs.embargo.termsNo embargoen_US
icr.researchteamDevelopment & Canceren_US
icr.researchteamGene Functionen_US
dc.contributor.icrauthorCampbell, Jamesen_US
dc.contributor.icrauthorSwain, Amandaen_US
dc.contributor.icrauthorLord, Christopheren_US
dc.contributor.icrauthorPettitt, Stephenen_US
dc.contributor.icrauthorKrastev, Dragomiren_US
dc.contributor.icrauthorSong, Feifeien_US


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