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dc.contributor.authorTasoulis, M-K
dc.contributor.authorLivaditi, O
dc.contributor.authorStamatakos, M
dc.contributor.authorStefanaki, C
dc.contributor.authorStefanaki, C
dc.contributor.authorPaneris, P
dc.contributor.authorPrigouris, P
dc.contributor.authorFlevari, A
dc.contributor.authorGoutas, N
dc.contributor.authorVlachodimitropoulos, D
dc.contributor.authorVilliotou, V
dc.contributor.authorDouzinas, EE
dc.date.accessioned2022-05-16T14:56:35Z
dc.date.available2022-05-16T14:56:35Z
dc.identifier.citationThe Tohoku journal of experimental medicine, 2009, 219 (3), pp. 193 - 199en
dc.identifier.issn0040-8727
dc.identifier.urihttps://repository.icr.ac.uk/handle/internal/5127
dc.identifier.eissn1349-3329en_US
dc.identifier.eissn1349-3329
dc.identifier.doi10.1620/tjem.219.193en_US
dc.identifier.doi10.1620/tjem.219.193
dc.description.abstractIncreased levels of cytokines or reactive oxygen species (ROS) in the bronchoalveolar lavage (BAL) fluid are associated with acute lung injury after ischemia/reperfusion. We investigated the correlation of these markers with the degree of lung injury in a rabbit model of hemorrhagic shock. Rabbits, maintained by mechanical ventilation, were left untreated (control) or subjected to hemorrhagic shock by withdrawing blood (n = 12 for each group). Shock animals were re-infused their shed blood for resuscitation. At the end of the experiment, BAL fluid was recovered, in which parameters of oxidative stress and cytokines were measured. Macrophages and malondialdehyde levels were increased (p = 0.043 and p = 0.003, respectively), and total antioxidant capacity (TAC) was decreased in the shock animals compared with control (p = 0.009). Production of ROS was significantly enhanced in shock animals compared with controls (p < 0.001). BAL fluid levels of tumor necrosis factor-alpha, interleukin (IL)-1beta and IL-6 were higher in shock rabbits by more than twofold (p < 0.001 for each). Shock animals also showed higher histopathological scores that represent severe tissue damage than controls (p = 0.022). Numbers of macrophages and levels of ROS and TAC were correlated with the degree of lung injury (p = 0.006, p = 0.02, and p = 0.04, respectively), but not cytokines. Therefore, resuscitation from hemorrhagic shock results in acute lung injury, with enhanced pulmonary oxidative and inflammatory responses. In conclusion, ROS in the BAL fluid are good markers that predict lung injury following hemorrhagic shock and resuscitation.en_US
dc.formatPrinten_US
dc.format.extent193 - 199en_US
dc.languageengen_US
dc.language.isoengen
dc.subjectBronchoalveolar Lavage Fluiden_US
dc.subjectAnimalsen_US
dc.subjectRabbitsen_US
dc.subjectShock, Hemorrhagicen_US
dc.subjectReactive Oxygen Speciesen_US
dc.subjectCytokinesen_US
dc.subjectResuscitationen_US
dc.subjectFluorescenceen_US
dc.subjectMaleen_US
dc.subjectLung Injuryen_US
dc.titleHigh concentrations of reactive oxygen species in the BAL fluid are correlated with lung injury in rabbits after hemorrhagic shock and resuscitation.en
dc.typeJournal Article
dcterms.dateAccepted2009-08-31
rioxxterms.versionNAen
rioxxterms.versionofrecord10.1620/tjem.219.193en
dc.relation.isPartOfThe Tohoku journal of experimental medicineen_US
pubs.issue3en_US
pubs.notesNo embargoen_US
pubs.organisational-group/ICR
pubs.publication-statusPublisheden_US
pubs.volume219en_US
pubs.embargo.termsNo embargoen_US
dc.contributor.icrauthorTasoulis, Mariosen_US


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