dc.contributor.author | Michelozzi, IM | |
dc.contributor.author | Sufi, J | |
dc.contributor.author | Adejumo, TA | |
dc.contributor.author | Amrolia, PJ | |
dc.contributor.author | Tape, CJ | |
dc.contributor.author | Giustacchini, A | |
dc.date.accessioned | 2022-05-27T09:27:03Z | |
dc.date.available | 2022-05-27T09:27:03Z | |
dc.identifier.citation | STAR protocols, 2022, 3 (1), pp. 101174 - ? | en |
dc.identifier.issn | 2666-1667 | |
dc.identifier.uri | https://repository.icr.ac.uk/handle/internal/5153 | |
dc.identifier.eissn | 2666-1667 | en_US |
dc.identifier.eissn | 2666-1667 | |
dc.identifier.doi | 10.1016/j.xpro.2022.101174 | en_US |
dc.identifier.doi | 10.1016/j.xpro.2022.101174 | |
dc.description.abstract | Here, we present a comprehensive protocol for the generation and functional characterization of chimeric antigen receptor (CAR) T cells and their products by mass cytometry in a reproducible and scalable manner. We describe the production of CAR T cells from human peripheral blood mononuclear cells. We then detail a three-step staining protocol with metal-labeled antibodies and the subsequent mass cytometry analysis. This protocol allows simultaneous characterization of CAR T cell intracellular signaling, activation, proliferation, cytokine production, and phenotype in a single assay. | en_US |
dc.format | Electronic-eCollection | en_US |
dc.format.extent | 101174 - ? | en_US |
dc.language | eng | en_US |
dc.language.iso | eng | en |
dc.rights.uri | https://creativecommons.org/licenses/by-nc-nd/4.0/ | en |
dc.subject | Leukocytes, Mononuclear | en_US |
dc.subject | T-Lymphocytes | en_US |
dc.subject | Humans | en_US |
dc.subject | Antibodies | en_US |
dc.title | High-dimensional functional phenotyping of preclinical human CAR T cells using mass cytometry. | en |
dc.type | Journal Article | |
dcterms.dateAccepted | 2022-03-18 | |
rioxxterms.version | VoR | en |
rioxxterms.versionofrecord | 10.1016/j.xpro.2022.101174 | en |
rioxxterms.licenseref.uri | https://creativecommons.org/licenses/by-nc-nd/4.0 | en |
dc.relation.isPartOf | STAR protocols | en_US |
pubs.issue | 1 | en_US |
pubs.notes | Not known | en_US |
pubs.organisational-group | /ICR | |
pubs.organisational-group | /ICR/Primary Group | |
pubs.organisational-group | /ICR/Primary Group/ICR Divisions | |
pubs.organisational-group | /ICR/Primary Group/ICR Divisions/Closed research teams | |
pubs.organisational-group | /ICR/Primary Group/ICR Divisions/Closed research teams/Oncogene | |
pubs.publication-status | Published | en_US |
pubs.volume | 3 | en_US |
pubs.embargo.terms | Not known | en_US |
icr.researchteam | Oncogene | |
dc.contributor.icrauthor | Tape, Christopher | |