High-dimensional functional phenotyping of preclinical human CAR T cells using mass cytometry.
Abstract
Here, we present a comprehensive protocol for the generation and functional characterization of chimeric antigen receptor (CAR) T cells and their products by mass cytometry in a reproducible and scalable manner. We describe the production of CAR T cells from human peripheral blood mononuclear cells. We then detail a three-step staining protocol with metal-labeled antibodies and the subsequent mass cytometry analysis. This protocol allows simultaneous characterization of CAR T cell intracellular signaling, activation, proliferation, cytokine production, and phenotype in a single assay.
Collections
Subject
Leukocytes, Mononuclear
T-Lymphocytes
Humans
Antibodies
Research team
Oncogene
Language
eng
Date accepted
2022-03-18
Citation
STAR protocols, 2022, 3 (1), pp. 101174 - ?