Bidirectional eukaryotic DNA replication is established by quasi-symmetrical helicase loading.
Date
2017-07-21ICR Author
Author
Coster, G
Diffley, JFX
Type
Journal Article
Metadata
Show full item recordAbstract
Bidirectional replication from eukaryotic DNA replication origins requires the loading of two ring-shaped minichromosome maintenance (MCM) helicases around DNA in opposite orientations. MCM loading is orchestrated by binding of the origin recognition complex (ORC) to DNA, but how ORC coordinates symmetrical MCM loading is unclear. We used natural budding yeast DNA replication origins and synthetic DNA sequences to show that efficient MCM loading requires binding of two ORC molecules to two ORC binding sites. The relative orientation of these sites, but not the distance between them, was found to be critical for MCM loading in vitro and origin function in vivo. We propose that quasi-symmetrical loading of individual MCM hexamers by ORC and directed MCM translocation into double hexamers acts as a unifying mechanism for the establishment of bidirectional replication in archaea and eukaryotes.
Collections
Subject
Binding Sites
DNA Helicases
DNA Replication
Minichromosome Maintenance Proteins
Origin Recognition Complex
Replication Origin
Saccharomyces cerevisiae
Saccharomyces cerevisiae Proteins
Research team
Genome Replication
Language
eng
Date accepted
2017-06-22
License start date
2017-07-21
Citation
Science, 2017, 357 (6348), pp. 314 - 318
Publisher
AMER ASSOC ADVANCEMENT SCIENCE