Rapid autopsies to enhance metastatic research: the UPTIDER post-mortem tissue donation program.
Date
2024-04-24ICR Author
Author
Geukens, T
De Schepper, M
Van Den Bogaert, W
Van Baelen, K
Maetens, M
Pabba, A
Mahdami, A
Leduc, S
Isnaldi, E
Nguyen, H-L
Bachir, I
Hajipirloo, M
Zels, G
Van Cauwenberge, J
Borremans, K
Vandecaveye, V
Weynand, B
Vermeulen, P
Leucci, E
Baietti, MF
Sflomos, G
Battista, L
Brisken, C
Derksen, PWB
Koorman, T
Visser, D
Scheele, CLGJ
Thommen, DS
Hatse, S
Fendt, S-M
Vanderheyden, E
Van Brussel, T
Schepers, R
Boeckx, B
Lambrechts, D
Marano, G
Biganzoli, E
Smeets, A
Nevelsteen, I
Punie, K
Neven, P
Wildiers, H
Richard, F
Floris, G
Desmedt, C
Type
Journal Article
Metadata
Show full item recordAbstract
Research on metastatic cancer has been hampered by limited sample availability. Here we present the breast cancer post-mortem tissue donation program UPTIDER and show how it enabled sampling of a median of 31 (range: 5-90) metastases and 5-8 liquids per patient from its first 20 patients. In a dedicated experiment, we show the mild impact of increasing time after death on RNA quality, transcriptional profiles and immunohistochemical staining in tumor tissue samples. We show that this impact can be counteracted by organ cooling. We successfully generated ex vivo models from tissue and liquid biopsies from distinct histological subtypes of breast cancer. We anticipate these and future findings of UPTIDER to elucidate mechanisms of disease progression and treatment resistance and to provide tools for the exploration of precision medicine strategies in the metastatic setting.
Collections
Subject
Science & Technology
Life Sciences & Biomedicine
Oncology
PROGESTERONE-RECEPTOR
GENE-EXPRESSION
PRIMARY TUMORS
COLD ISCHEMIA
BREAST
HETEROGENEITY
PROCUREMENT
ESTROGEN
MODEL
TIME
Research team
Endocrine control mechans
Language
eng
Date accepted
2024-04-05
License start date
2024-04-24
Citation
npj Breast Cancer, 2024, 10 (1), pp. 31 -
Publisher
NATURE PORTFOLIO