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dc.contributor.authorDi Fiore, Ben_US
dc.contributor.authorWurzenberger, Cen_US
dc.contributor.authorDavey, NEen_US
dc.contributor.authorPines, Jen_US
dc.date.accessioned2017-10-31T15:51:45Z
dc.date.issued2016-12-08en_US
dc.identifier.citationMolecular cell, 2016, 64 (6), pp. 1144 - 1153en_US
dc.identifier.issn1097-2765en_US
dc.identifier.urihttps://repository.icr.ac.uk/handle/internal/881
dc.identifier.eissn1097-4164en_US
dc.identifier.doi10.1016/j.molcel.2016.11.006en_US
dc.description.abstractThe Spindle Assembly Checkpoint (SAC) ensures genomic stability by preventing sister chromatid separation until all chromosomes are attached to the spindle. It catalyzes the production of the Mitotic Checkpoint Complex (MCC), which inhibits Cdc20 to inactivate the Anaphase Promoting Complex/Cyclosome (APC/C). Here we show that two Cdc20-binding motifs in BubR1 of the recently identified ABBA motif class are crucial for the MCC to recognize active APC/C-Cdc20. Mutating these motifs eliminates MCC binding to the APC/C, thereby abolishing the SAC and preventing cells from arresting in response to microtubule poisons. These ABBA motifs flank a KEN box to form a cassette that is highly conserved through evolution, both in the arrangement and spacing of the ABBA-KEN-ABBA motifs, and association with the amino-terminal KEN box required to form the MCC. We propose that the ABBA-KEN-ABBA cassette holds the MCC onto the APC/C by binding the two Cdc20 molecules in the MCC-APC/C complex.en_US
dc.formatPrint-Electronicen_US
dc.format.extent1144 - 1153en_US
dc.languageengen_US
dc.language.isoengen_US
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/en_US
dc.subjectHela Cellsen_US
dc.subjectAnimalsen_US
dc.subjectCiona intestinalisen_US
dc.subjectHumansen_US
dc.subjectDrosophila melanogasteren_US
dc.subjectCaenorhabditis elegansen_US
dc.subjectArabidopsisen_US
dc.subjectProtein-Serine-Threonine Kinasesen_US
dc.subjectSequence Alignmenten_US
dc.subjectGene Expressionen_US
dc.subjectAmino Acid Motifsen_US
dc.subjectConserved Sequenceen_US
dc.subjectSequence Homology, Amino Aciden_US
dc.subjectMutationen_US
dc.subjectBiological Evolutionen_US
dc.subjectTime-Lapse Imagingen_US
dc.subjectM Phase Cell Cycle Checkpointsen_US
dc.subjectAnaphase-Promoting Complex-Cyclosomeen_US
dc.subjectCdc20 Proteinsen_US
dc.titleThe Mitotic Checkpoint Complex Requires an Evolutionary Conserved Cassette to Bind and Inhibit Active APC/C.en_US
dc.typeJournal Article
dcterms.dateAccepted2016-10-31en_US
rioxxterms.versionofrecord10.1016/j.molcel.2016.11.006en_US
rioxxterms.licenseref.urihttps://creativecommons.org/licenses/by/4.0en_US
rioxxterms.licenseref.startdate2016-12-08en_US
rioxxterms.typeJournal Article/Reviewen_US
dc.relation.isPartOfMolecular cellen_US
pubs.issue6en_US
pubs.notes6 monthsen_US
pubs.organisational-group/ICR
pubs.publication-statusPublisheden_US
pubs.volume64en_US
pubs.embargo.terms6 monthsen_US
dc.contributor.icrauthorPines, Jonathonen_US
dc.contributor.icrauthorDavey, Normanen_US


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