Show simple item record

dc.contributor.authorDi Fiore, B
dc.contributor.authorWurzenberger, C
dc.contributor.authorDavey, NE
dc.contributor.authorPines, J
dc.date.accessioned2017-10-31T15:51:45Z
dc.date.issued2016-12-08
dc.identifier.citationMolecular cell, 2016, 64 (6), pp. 1144 - 1153
dc.identifier.issn1097-2765
dc.identifier.urihttps://repository.icr.ac.uk/handle/internal/881
dc.identifier.eissn1097-4164
dc.identifier.doi10.1016/j.molcel.2016.11.006
dc.description.abstractThe Spindle Assembly Checkpoint (SAC) ensures genomic stability by preventing sister chromatid separation until all chromosomes are attached to the spindle. It catalyzes the production of the Mitotic Checkpoint Complex (MCC), which inhibits Cdc20 to inactivate the Anaphase Promoting Complex/Cyclosome (APC/C). Here we show that two Cdc20-binding motifs in BubR1 of the recently identified ABBA motif class are crucial for the MCC to recognize active APC/C-Cdc20. Mutating these motifs eliminates MCC binding to the APC/C, thereby abolishing the SAC and preventing cells from arresting in response to microtubule poisons. These ABBA motifs flank a KEN box to form a cassette that is highly conserved through evolution, both in the arrangement and spacing of the ABBA-KEN-ABBA motifs, and association with the amino-terminal KEN box required to form the MCC. We propose that the ABBA-KEN-ABBA cassette holds the MCC onto the APC/C by binding the two Cdc20 molecules in the MCC-APC/C complex.
dc.formatPrint-Electronic
dc.format.extent1144 - 1153
dc.languageeng
dc.language.isoeng
dc.rights.urihttps://creativecommons.org/licenses/by/4.0
dc.subjectHela Cells
dc.subjectAnimals
dc.subjectCiona intestinalis
dc.subjectHumans
dc.subjectDrosophila melanogaster
dc.subjectCaenorhabditis elegans
dc.subjectArabidopsis
dc.subjectProtein-Serine-Threonine Kinases
dc.subjectSequence Alignment
dc.subjectGene Expression
dc.subjectAmino Acid Motifs
dc.subjectConserved Sequence
dc.subjectSequence Homology, Amino Acid
dc.subjectMutation
dc.subjectBiological Evolution
dc.subjectTime-Lapse Imaging
dc.subjectM Phase Cell Cycle Checkpoints
dc.subjectAnaphase-Promoting Complex-Cyclosome
dc.subjectCdc20 Proteins
dc.titleThe Mitotic Checkpoint Complex Requires an Evolutionary Conserved Cassette to Bind and Inhibit Active APC/C.
dc.typeJournal Article
dcterms.dateAccepted2016-10-31
rioxxterms.versionofrecord10.1016/j.molcel.2016.11.006
rioxxterms.licenseref.urihttps://creativecommons.org/licenses/by/4.0
rioxxterms.licenseref.startdate2016-12-08
rioxxterms.typeJournal Article/Review
dc.relation.isPartOfMolecular cell
pubs.issue6
pubs.notes6 months
pubs.organisational-group/ICR
pubs.organisational-group/ICR
pubs.publication-statusPublished
pubs.volume64
pubs.embargo.terms6 months
dc.contributor.icrauthorPines, Jonathonen
dc.contributor.icrauthorDavey, Normanen


Files in this item

Thumbnail

This item appears in the following collection(s)

Show simple item record

https://creativecommons.org/licenses/by/4.0
Except where otherwise noted, this item's license is described as https://creativecommons.org/licenses/by/4.0