Browsing Structural Biology by author "Vannini, Alessandro"
Now showing items 1-15 of 15
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A commercial antibody to the human condensin II subunit NCAPH2 cross-reacts with a SWI/SNF complex component
Cutts, E; Taylor, G; Pardo, M; Yu, L; Wills, J; et al. (2021-01-13)Condensin complexes compact and disentangle chromosomes in preparation for cell division. Commercially available antibodies raised against condensin subunits have been widely used to characterise their cellular interactome. ... -
A commercial antibody to the human condensin II subunit NCAPH2 cross-reacts with a SWI/SNF complex component.
Cutts, EE; Taylor, GC; Pardo, M; Yu, L; Wills, JC; et al. (F1000 Research Ltd, 2021-01-13)Condensin complexes compact and disentangle chromosomes in preparation for cell division. Commercially available antibodies raised against condensin subunits have been widely used to characterise their cellular interactome. ... -
DNA origami-based single-molecule force spectroscopy elucidates RNA Polymerase III pre-initiation complex stability.
Kramm, K; Schröder, T; Gouge, J; Vera, AM; Gupta, K; et al. (NATURE PUBLISHING GROUP, 2020-06-05)The TATA-binding protein (TBP) and a transcription factor (TF) IIB-like factor are important constituents of all eukaryotic initiation complexes. The reason for the emergence and strict requirement of the additional ... -
Human Condensin I and II Drive Extensive ATP-Dependent Compaction of Nucleosome-Bound DNA.
Kong, M; Cutts, EE; Pan, D; Beuron, F; Kaliyappan, T; et al. (CELL PRESS, 2020-07-02)Structural maintenance of chromosomes (SMC) complexes are essential for genome organization from bacteria to humans, but their mechanisms of action remain poorly understood. Here, we characterize human SMC complexes condensin ... -
Hybrid Gene Origination Creates Human-Virus Chimeric Proteins during Infection.
Ho, JSY; Angel, M; Ma, Y; Sloan, E; Wang, G; et al. (CELL PRESS, 2020-06-25)RNA viruses are a major human health threat. The life cycles of many highly pathogenic RNA viruses like influenza A virus (IAV) and Lassa virus depends on host mRNA, because viral polymerases cleave 5'-m7G-capped host ... -
MCPH1 inhibits Condensin II during interphase by regulating its SMC2-Kleisin interface.
Houlard, M; Cutts, EE; Shamim, MS; Godwin, J; Weisz, D; et al. (eLife Sciences Publications, Ltd, 2021-12-01)Dramatic change in chromosomal DNA morphology between interphase and mitosis is a defining features of the eukaryotic cell cycle. Two types of enzymes, namely cohesin and condensin confer the topology of chromosomal DNA ... -
Mechanism of selective recruitment of RNA polymerases II and III to snRNA gene promoters.
Dergai, O; Cousin, P; Gouge, J; Satia, K; Praz, V; et al. (COLD SPRING HARBOR LAB PRESS, PUBLICATIONS DEPT, 2018-05-01)RNA polymerase II (Pol II) small nuclear RNA (snRNA) promoters and type 3 Pol III promoters have highly similar structures; both contain an interchangeable enhancer and "proximal sequence element" (PSE), which recruits the ... -
Molecular mechanisms of Bdp1 in TFIIIB assembly and RNA polymerase III transcription initiation.
Gouge, J; Guthertz, N; Kramm, K; Dergai, O; Abascal-Palacios, G; et al. (NATURE PUBLISHING GROUP, 2017-07-25)Initiation of gene transcription by RNA polymerase (Pol) III requires the activity of TFIIIB, a complex formed by Brf1 (or Brf2), TBP (TATA-binding protein), and Bdp1. TFIIIB is required for recruitment of Pol III and to ... -
New tricks for an old dog: Brf2-dependent RNA Polymerase III transcription in oxidative stress and cancer.
Gouge, J; Vannini, A (TAYLOR & FRANCIS INC, 2018-01-01)Here, we discuss the role of Brf2, an RNA Polymerase III core transcription factor, as a master switch of the oxidative stress response. We highlight the interplay of Brf2 with the Nrf2/Keap1 pathway, as well as the role ... -
Redox Signaling by the RNA Polymerase III TFIIB-Related Factor Brf2.
Gouge, J; Satia, K; Guthertz, N; Widya, M; Thompson, AJ; et al. (CELL PRESS, 2015-12-03)TFIIB-related factor 2 (Brf2) is a member of the family of TFIIB-like core transcription factors. Brf2 recruits RNA polymerase (Pol) III to type III gene-external promoters, including the U6 spliceosomal RNA and selenocysteine ... -
RNA polymerase I, bending the rules?
Jochem, L; Ramsay, EP; Vannini, A (WILEY, 2017-09-15)Transcription initiation is one of the key regulatory steps in expressing the genetic information encoded in the DNA. Mechanisms of RNA Pol II transcription have been extensively studied, whereas the structural basis of ... -
Structural basis of RNA polymerase III transcription initiation.
Abascal-Palacios, G; Ramsay, EP; Beuron, F; Morris, E; Vannini, A (NATURE PORTFOLIO, 2018-01-17)RNA polymerase (Pol) III transcribes essential non-coding RNAs, including the entire pool of transfer RNAs, the 5S ribosomal RNA and the U6 spliceosomal RNA, and is often deregulated in cancer cells. The initiation of gene ... -
Structural basis of Ty3 retrotransposon integration at RNA Polymerase III-transcribed genes.
Abascal-Palacios, G; Jochem, L; Pla-Prats, C; Beuron, F; Vannini, A (NATURE PORTFOLIO, 2021-11-30)Retrotransposons are endogenous elements that have the ability to mobilise their DNA between different locations in the host genome. The Ty3 retrotransposon integrates with an exquisite specificity in a narrow window ... -
Structural rearrangements of the RNA polymerase III machinery during tRNA transcription initiation.
Ramsay, EP; Vannini, A (ELSEVIER SCIENCE BV, 2018-04-01)RNA polymerase III catalyses the synthesis of tRNAs in eukaryotic organisms. Through combined biochemical and structural characterisation, multiple auxiliary factors have been identified alongside RNA Polymerase III as ... -
Structure of human RNA polymerase III.
Ramsay, EP; Abascal-Palacios, G; Daiß, JL; King, H; Gouge, J; et al. (NATURE RESEARCH, 2020-12-17)In eukaryotes, RNA Polymerase (Pol) III is specialized for the transcription of tRNAs and other short, untranslated RNAs. Pol III is a determinant of cellular growth and lifespan across eukaryotes. Upregulation of Pol III ...