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dc.contributor.authorRanftl, RE
dc.contributor.authorCalvo, F
dc.date.accessioned2017-11-22T10:20:59Z
dc.date.issued2017-01
dc.identifier.citationMethods in molecular biology (Clifton, N.J.), 2017, 1612 pp. 199 - 212
dc.identifier.issn1064-3745
dc.identifier.urihttps://repository.icr.ac.uk/handle/internal/917
dc.identifier.eissn1940-6029
dc.identifier.doi10.1007/978-1-4939-7021-6_15
dc.description.abstractMetastasis is the main cause of cancer patient mortality. Local tumor invasion is a key step in metastatic dissemination whereby cancer cells dislodge from primary tumors, migrate through the peritumoral stroma and reach the circulation. This is a highly dynamic process occurring in three dimensions that involves interactions between tumor, stromal cells, and the extracellular matrix. Here we describe the organotypic culture system and its utility to study breast cancer cell invasion induced by cancer-associated fibroblasts. This is a three-dimensional model that reproduces the biochemical and physiological properties of real tissue and allows for investigating the molecular and cellular mechanisms involving tumor and its microenvironment, and their contribution to cancer cell invasion . This system provides a robust, accurate, and reproducible method for measuring cancer cell invasion and represents a valuable tool to improve the mechanistic understanding of the initial steps in metastasis .
dc.formatPrint
dc.format.extent199 - 212
dc.languageeng
dc.language.isoeng
dc.rights.urihttps://creativecommons.org/licenses/by/4.0
dc.subjectOrganoids
dc.subjectCell Line, Tumor
dc.subjectFibroblasts
dc.subjectStromal Cells
dc.subjectAnimals
dc.subjectHumans
dc.subjectMice
dc.subjectBreast Neoplasms
dc.subjectNeoplasm Invasiveness
dc.subjectCoculture Techniques
dc.subjectFemale
dc.subjectTumor Microenvironment
dc.subjectCancer-Associated Fibroblasts
dc.titleAnalysis of Breast Cancer Cell Invasion Using an Organotypic Culture System.
dc.typeJournal Article
dcterms.dateAccepted2017-02-01
rioxxterms.versionofrecord10.1007/978-1-4939-7021-6_15
rioxxterms.licenseref.urihttps://creativecommons.org/licenses/by/4.0
rioxxterms.licenseref.startdate2017-01
rioxxterms.typeJournal Article/Review
dc.relation.isPartOfMethods in molecular biology (Clifton, N.J.)
pubs.notesNot known
pubs.organisational-group/ICR
pubs.organisational-group/ICR/Primary Group
pubs.organisational-group/ICR/Primary Group/ICR Divisions
pubs.organisational-group/ICR/Primary Group/ICR Divisions/Closed research teams
pubs.organisational-group/ICR/Primary Group/ICR Divisions/Closed research teams/Tumour Microenvironment
pubs.organisational-group/ICR
pubs.organisational-group/ICR/Primary Group
pubs.organisational-group/ICR/Primary Group/ICR Divisions
pubs.organisational-group/ICR/Primary Group/ICR Divisions/Closed research teams
pubs.organisational-group/ICR/Primary Group/ICR Divisions/Closed research teams/Tumour Microenvironment
pubs.publication-statusPublished
pubs.volume1612
pubs.embargo.termsNot known
icr.researchteamTumour Microenvironmenten_US
dc.contributor.icrauthorCalvo, Fernando


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