Analysis of Breast Cancer Cell Invasion Using an Organotypic Culture System.
View/ Open
Date
2017-01ICR Author
Author
Ranftl, RE
Calvo, F
Type
Journal Article
Metadata
Show full item recordAbstract
Metastasis is the main cause of cancer patient mortality. Local tumor invasion is a key step in metastatic dissemination whereby cancer cells dislodge from primary tumors, migrate through the peritumoral stroma and reach the circulation. This is a highly dynamic process occurring in three dimensions that involves interactions between tumor, stromal cells, and the extracellular matrix. Here we describe the organotypic culture system and its utility to study breast cancer cell invasion induced by cancer-associated fibroblasts. This is a three-dimensional model that reproduces the biochemical and physiological properties of real tissue and allows for investigating the molecular and cellular mechanisms involving tumor and its microenvironment, and their contribution to cancer cell invasion . This system provides a robust, accurate, and reproducible method for measuring cancer cell invasion and represents a valuable tool to improve the mechanistic understanding of the initial steps in metastasis .
Collections
Subject
Organoids
Cell Line, Tumor
Fibroblasts
Stromal Cells
Animals
Humans
Mice
Breast Neoplasms
Neoplasm Invasiveness
Coculture Techniques
Female
Tumor Microenvironment
Cancer-Associated Fibroblasts
Research team
Tumour Microenvironment
Language
eng
Date accepted
2017-02-01
License start date
2017-01
Citation
Methods in molecular biology (Clifton, N.J.), 2017, 1612 pp. 199 - 212