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A Pocket on the Surface of the N-Terminal BRCT Domain of Mcph1 Is Required to Prevent Abnormal Chromosome Condensation

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Publication Date
2010-02-05
ICR Author
Bayliss, Richard
Author
Richards, MW
Leung, JWC
Roe, SM
Li, K
Chen, J
Bayliss, R
Type
Journal Article
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Abstract
Mcph1 is mutated in autosomal recessive primary microcephaly and premature chromosome condensation (PCC) syndrome. Increased chromosome condensation is a common feature of cells isolated from patients afflicted with either disease. Normal cells depleted of Mcph1 also exhibit PCC phenotype. Human Mcph1 contains three BRCA1-carboxyl terminal (BRCT) domains, the first of which (Mcph1N) is necessary for the prevention of PCC. The only known disease-associated missense mutation in Mcph1 resides in this domain (T27R). We have determined the X-ray crystal structure of human Mcph1N to 16 angstrom resolution Compared with other BRCT domain structures, the most striking differences are an elongated, ordered beta 1-alpha 1 loop and an adjacent hydrophobic pocket This pocket is in the equivalent structural position to the phosphate binding site of BRCT domains that recognize phospho-proteins, although the phosphate-binding residues are absent in Mcph1N. Mutations in the pocket abrogate the ability of full-length Mcph1 to rescue the PCC phenotype of Mcph1(-/) mouse embryonic fibroblast cells, suggesting that it forms an essential part of a protein-protein interaction site necessary to prevent PCC. (C) 2009 Elsevier Ltd. All rights reserved
URL
https://repository.icr.ac.uk/handle/internal/2380
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Version of record
10.1016/j.jmb.2009.11.029
Language
English
License start date
2010-02-05
Citation
JOURNAL OF MOLECULAR BIOLOGY, 2010, 395 pp. 908 - 915
Publisher
ACADEMIC PRESS LTD- ELSEVIER SCIENCE LTD

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