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Now showing items 11-20 of 36
A comprehensive model for the proliferation-quiescence decision in response to endogenous DNA damage in human cells.
(NATL ACAD SCIENCES, 2018-03-06)
Human cells that suffer mild DNA damage can enter a reversible state of growth arrest known as quiescence. This decision to temporarily exit the cell cycle is essential to prevent the propagation of mutations, and most ...
Squeezing through the microcirculation: survival adaptations of circulating tumour cells to seed metastasis.
(SPRINGERNATURE, 2021-01-05)
During metastasis, tumour cells navigating the vascular circulatory system-circulating tumour cells (CTCs)-encounter capillary beds, where they start the process of extravasation. Biomechanical constriction forces exerted ...
ALPK1 hotspot mutation as a driver of human spiradenoma and spiradenocarcinoma.
(NATURE PUBLISHING GROUP, 2019-05-17)
Spiradenoma and cylindroma are distinctive skin adnexal tumors with sweat gland differentiation and potential for malignant transformation and aggressive behaviour. We present the genomic analysis of 75 samples from 57 ...
Robust deep learning optical autofocus system applied to automated multiwell plate single molecule localization microscopy.
(WILEY, 2021-06-04)
We presenta robust, long-range optical autofocus system for microscopy utilizing machine learning. This can be useful for experiments with long image data acquisition times that may be impacted by defocusing resulting from ...
Cyclin A triggers Mitosis either via the Greatwall kinase pathway or Cyclin B.
(WILEY, 2020-06-02)
Two mitotic cyclin types, cyclin A and B, exist in higher eukaryotes, but their specialised functions in mitosis are incompletely understood. Using degron tags for rapid inducible protein removal, we analyse how acute ...
Voltage imaging reveals the dynamic electrical signatures of human breast cancer cells.
(NATURE PORTFOLIO, 2022-11-11)
Cancer cells feature a resting membrane potential (Vm) that is depolarized compared to normal cells, and express active ionic conductances, which factor directly in their pathophysiological behavior. Despite similarities ...
Proteomic characterisation of triple negative breast cancer cells following CDK4/6 inhibition.
(NATURE PORTFOLIO, 2022-07-11)
When used in combination with hormone treatment, Palbociclib prolongs progression-free survival of patients with hormone receptor positive breast cancer. Mechanistically, Palbociclib inhibits CDK4/6 activity but the basis ...
Arresting metastasis within the microcirculation.
(SPRINGER, 2021-07-09)
The behaviour of circulating tumour cells in the microcirculation remains poorly understood. Growing evidence suggests that biomechanical adaptations and interactions with blood components, i.e. immune cells and platelets ...
ARHGEF9 regulates melanoma morphogenesis in environments with diverse geometry and elasticity by promoting filopodial-driven adhesion.
(CELL PRESS, 2022-08-19)
Rho GTP Exchange Factors (RhoGEFs) and Rho GTPase Activating Proteins (RhoGAPs) are large families of molecules that regulate shape determination in all eukaryotes. In pathologies such as melanoma, RhoGEF and RhoGAP activity ...
Accelerating Live Single-Cell Signalling Studies.
(ELSEVIER SCIENCE LONDON, 2017-05-01)
The dynamics of signalling networks that couple environmental conditions with cellular behaviour can now be characterised in exquisite detail using live single-cell imaging experiments. Recent improvements in our abilities ...