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dc.contributor.authorGreber, BJ
dc.contributor.authorGerhardy, S
dc.contributor.authorLeitner, A
dc.contributor.authorLeibundgut, M
dc.contributor.authorSalem, M
dc.contributor.authorBoehringer, D
dc.contributor.authorLeulliot, N
dc.contributor.authorAebersold, R
dc.contributor.authorPanse, VG
dc.contributor.authorBan, N
dc.date.accessioned2020-10-05T09:36:56Z
dc.date.issued2016-01
dc.identifier.citationCell, 2016, 164 (1-2), pp. 91 - 102
dc.identifier.issn0092-8674
dc.identifier.urihttps://repository.icr.ac.uk/handle/internal/4125
dc.identifier.eissn1097-4172
dc.identifier.doi10.1016/j.cell.2015.11.027
dc.description.abstractEukaryotic ribosome biogenesis depends on several hundred assembly factors to produce functional 40S and 60S ribosomal subunits. The final phase of 60S subunit biogenesis is cytoplasmic maturation, which includes the proofreading of functional centers of the 60S subunit and the release of several ribosome biogenesis factors. We report the cryo-electron microscopy (cryo-EM) structure of the yeast 60S subunit in complex with the biogenesis factors Rei1, Arx1, and Alb1 at 3.4 Å resolution. In addition to the network of interactions formed by Alb1, the structure reveals a mechanism for ensuring the integrity of the ribosomal polypeptide exit tunnel. Arx1 probes the entire set of inner-ring proteins surrounding the tunnel exit, and the C terminus of Rei1 is deeply inserted into the ribosomal tunnel, where it forms specific contacts along almost its entire length. We provide genetic and biochemical evidence that failure to insert the C terminus of Rei1 precludes subsequent steps of 60S maturation.
dc.formatPrint-Electronic
dc.format.extent91 - 102
dc.languageeng
dc.language.isoeng
dc.rights.urihttps://www.rioxx.net/licenses/all-rights-reserved
dc.subjectHumans
dc.subjectSaccharomyces cerevisiae
dc.subjectChaetomium
dc.subjectFungal Proteins
dc.subjectSaccharomyces cerevisiae Proteins
dc.subjectRibosomal Proteins
dc.subjectCryoelectron Microscopy
dc.subjectSequence Alignment
dc.subjectAmino Acid Sequence
dc.subjectProtein Structure, Tertiary
dc.subjectModels, Chemical
dc.subjectModels, Molecular
dc.subjectMolecular Sequence Data
dc.subjectRibosome Subunits, Large, Eukaryotic
dc.titleInsertion of the Biogenesis Factor Rei1 Probes the Ribosomal Tunnel during 60S Maturation.
dc.typeJournal Article
dcterms.dateAccepted2015-11-05
rioxxterms.versionofrecord10.1016/j.cell.2015.11.027
rioxxterms.licenseref.urihttps://www.rioxx.net/licenses/all-rights-reserved
rioxxterms.licenseref.startdate2016-01
rioxxterms.typeJournal Article/Review
dc.relation.isPartOfCell
pubs.issue1-2
pubs.notesNo embargo
pubs.organisational-group/ICR
pubs.organisational-group/ICR
pubs.publication-statusPublished
pubs.volume164
pubs.embargo.termsNo embargo
dc.contributor.icrauthorGreber, Basilen


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