Insertion of the Biogenesis Factor Rei1 Probes the Ribosomal Tunnel during 60S Maturation.
Date
2016-01-14ICR Author
Author
Greber, BJ
Gerhardy, S
Leitner, A
Leibundgut, M
Salem, M
Boehringer, D
Leulliot, N
Aebersold, R
Panse, VG
Ban, N
Type
Journal Article
Metadata
Show full item recordAbstract
Eukaryotic ribosome biogenesis depends on several hundred assembly factors to produce functional 40S and 60S ribosomal subunits. The final phase of 60S subunit biogenesis is cytoplasmic maturation, which includes the proofreading of functional centers of the 60S subunit and the release of several ribosome biogenesis factors. We report the cryo-electron microscopy (cryo-EM) structure of the yeast 60S subunit in complex with the biogenesis factors Rei1, Arx1, and Alb1 at 3.4 Å resolution. In addition to the network of interactions formed by Alb1, the structure reveals a mechanism for ensuring the integrity of the ribosomal polypeptide exit tunnel. Arx1 probes the entire set of inner-ring proteins surrounding the tunnel exit, and the C terminus of Rei1 is deeply inserted into the ribosomal tunnel, where it forms specific contacts along almost its entire length. We provide genetic and biochemical evidence that failure to insert the C terminus of Rei1 precludes subsequent steps of 60S maturation.
Collections
Subject
Humans
Saccharomyces cerevisiae
Chaetomium
Fungal Proteins
Saccharomyces cerevisiae Proteins
Ribosomal Proteins
Cryoelectron Microscopy
Sequence Alignment
Amino Acid Sequence
Protein Structure, Tertiary
Models, Chemical
Models, Molecular
Molecular Sequence Data
Ribosome Subunits, Large, Eukaryotic
Language
eng
Date accepted
2015-11-05
License start date
2016-01
Citation
Cell, 2016, 164 (1-2), pp. 91 - 102
Publisher
CELL PRESS