Systematic evaluation of quantotypic peptides for targeted analysis of the human kinome.
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In targeted proteomics it is critical that peptides are not only proteotypic but also accurately represent the level of the protein (quantotypic). Numerous approaches are used to identify proteotypic peptides, but quantotypic properties are rarely assessed. We show that measuring ratios of proteotypic peptides across biological samples can be used to empirically identify peptides with good quantotypic properties. We applied this technique to identify quantotypic peptides for 21% of the human kinome.
Cell Line, Tumor
Gene Expression Regulation, Neoplastic
Computational Pathology & Integrated Genomics
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Nature methods, 2014, 11 (10), pp. 1041 - 1044