Systematic evaluation of quantotypic peptides for targeted analysis of the human kinome.
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Date
2014-10-01Author
Worboys, JD
Sinclair, J
Yuan, Y
Jørgensen, C
Type
Journal Article
Metadata
Show full item recordAbstract
In targeted proteomics it is critical that peptides are not only proteotypic but also accurately represent the level of the protein (quantotypic). Numerous approaches are used to identify proteotypic peptides, but quantotypic properties are rarely assessed. We show that measuring ratios of proteotypic peptides across biological samples can be used to empirically identify peptides with good quantotypic properties. We applied this technique to identify quantotypic peptides for 21% of the human kinome.
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Subject
Cell Line, Tumor
Humans
Protein Kinases
Peptides
Proteins
Proteome
Chromatography
Proteomics
Computational Biology
Gene Expression Regulation, Neoplastic
Algorithms
Research team
Cell Communication
Computational Pathology & Integrated Genomics
Language
eng
Date accepted
2014-07-14
License start date
2014-10
Citation
Nature methods, 2014, 11 (10), pp. 1041 - 1044
Publisher
NATURE PUBLISHING GROUP