Magnetic Resonance Spectroscopy (MRS)-Based Methods for Examining Cancer Metabolism in Response to Oncogenic Kinase Drug Treatment.
Abstract
Magnetic resonance spectroscopy (MRS) is an analytical technique that has been extensively used to examine reprogrammed metabolism and treatment response in cancer cells and solid tumors both in vivo and ex vivo. High-resolution MRS (HR-MRS) is one of the best methods for metabolic profiling, as it is highly quantitative, robust, and reproducible. The protocols for dual-phase extraction of cancer cells and tumors and sample preparations for high-resolution 1H and 31P HR-MRS analysis are described here. Descriptions of spectra acquisition and analysis are also included in this chapter.
Collections
Subject
Humans
Protein Kinases
Oncogene Proteins
Protein Kinase Inhibitors
Chemical Fractionation
Magnetic Resonance Spectroscopy
Energy Metabolism
Solubility
Lipid Metabolism
Metabolomics
Language
eng
Date accepted
2016-04-21
License start date
2017-01
Citation
Methods in molecular biology (Clifton, N.J.), 2017, 1636 pp. 393 - 404
Publisher
HUMANA PRESS INC