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dc.contributor.authorTodd, JR
dc.contributor.authorRyall, KA
dc.contributor.authorVyse, S
dc.contributor.authorWong, JP
dc.contributor.authorNatrajan, RC
dc.contributor.authorYuan, Y
dc.contributor.authorTan, A-C
dc.contributor.authorHuang, PH
dc.date.accessioned2016-08-22T14:53:37Z
dc.date.issued2016-09
dc.identifier.citationOncotarget, 2016, 7 (39), pp. 62939 - 62953
dc.identifier.issn1949-2553
dc.identifier.urihttps://repository.icr.ac.uk/handle/internal/70
dc.identifier.eissn1949-2553
dc.identifier.doi10.18632/oncotarget.11307
dc.description.abstractTumour cell-extracellular matrix (ECM) interactions are fundamental for discrete steps in breast cancer progression. In particular, cancer cell adhesion to ECM proteins present in the microenvironment is critical for accelerating tumour growth and facilitating metastatic spread. To assess the utility of tumour cell-ECM adhesion as a means for discovering prognostic factors in breast cancer survival, here we perform a systematic phenotypic screen and characterise the adhesion properties of a panel of human HER2 amplified breast cancer cell lines across six ECM proteins commonly deregulated in breast cancer. We determine a gene expression signature that defines a subset of cell lines displaying impaired adhesion to laminin. Cells with impaired laminin adhesion showed an enrichment in genes associated with cell motility and molecular pathways linked to cytokine signalling and inflammation. Evaluation of this gene set in the Molecular Taxonomy of Breast Cancer International Consortium (METABRIC) cohort of 1,964 patients identifies the F12 and STC2 genes as independent prognostic factors for overall survival in breast cancer. Our study demonstrates the potential of in vitro cell adhesion screens as a novel approach for identifying prognostic factors for disease outcome.
dc.formatPrint
dc.format.extent62939 - 62953
dc.languageeng
dc.language.isoeng
dc.rights.urihttps://creativecommons.org/licenses/by/4.0
dc.subjectExtracellular Matrix
dc.subjectHumans
dc.subjectBreast Neoplasms
dc.subjectNeoplasm Metastasis
dc.subjectDisease Progression
dc.subjectInflammation
dc.subjectReceptor, erbB-2
dc.subjectLaminin
dc.subjectCytokines
dc.subjectPrognosis
dc.subjectTreatment Outcome
dc.subjectTissue Array Analysis
dc.subjectCohort Studies
dc.subjectGene Expression Profiling
dc.subjectCell Adhesion
dc.subjectSignal Transduction
dc.subjectCell Proliferation
dc.subjectGene Expression Regulation, Neoplastic
dc.subjectFemale
dc.titleSystematic analysis of tumour cell-extracellular matrix adhesion identifies independent prognostic factors in breast cancer.
dc.typeJournal Article
dcterms.dateAccepted2016-07-27
rioxxterms.funderThe Institute of Cancer Research
rioxxterms.identifier.projectUnspecified
rioxxterms.versionofrecord10.18632/oncotarget.11307
rioxxterms.licenseref.urihttps://creativecommons.org/licenses/by/4.0
rioxxterms.licenseref.startdate2016-09
rioxxterms.typeJournal Article/Review
dc.relation.isPartOfOncotarget
pubs.issue39
pubs.notesNo embargo
pubs.organisational-group/ICR
pubs.organisational-group/ICR/Primary Group
pubs.organisational-group/ICR/Primary Group/ICR Divisions
pubs.organisational-group/ICR/Primary Group/ICR Divisions/Breast Cancer Research
pubs.organisational-group/ICR/Primary Group/ICR Divisions/Breast Cancer Research/Functional Genomics
pubs.organisational-group/ICR/Primary Group/ICR Divisions/Cancer Biology
pubs.organisational-group/ICR/Primary Group/ICR Divisions/Cancer Biology/Protein Networks
pubs.organisational-group/ICR/Primary Group/ICR Divisions/Molecular Pathology
pubs.organisational-group/ICR/Primary Group/ICR Divisions/Molecular Pathology/Functional Genomics
pubs.organisational-group/ICR/Primary Group/ICR Divisions/Molecular Pathology/Molecular and Systems Oncology
pubs.organisational-group/ICR
pubs.organisational-group/ICR/Primary Group
pubs.organisational-group/ICR/Primary Group/ICR Divisions
pubs.organisational-group/ICR/Primary Group/ICR Divisions/Breast Cancer Research
pubs.organisational-group/ICR/Primary Group/ICR Divisions/Breast Cancer Research/Functional Genomics
pubs.organisational-group/ICR/Primary Group/ICR Divisions/Cancer Biology
pubs.organisational-group/ICR/Primary Group/ICR Divisions/Cancer Biology/Protein Networks
pubs.organisational-group/ICR/Primary Group/ICR Divisions/Molecular Pathology
pubs.organisational-group/ICR/Primary Group/ICR Divisions/Molecular Pathology/Functional Genomics
pubs.organisational-group/ICR/Primary Group/ICR Divisions/Molecular Pathology/Molecular and Systems Oncology
pubs.publication-statusPublished
pubs.volume7
pubs.embargo.termsNo embargo
icr.researchteamProtein Networksen_US
icr.researchteamFunctional Genomicsen_US
icr.researchteamMolecular and Systems Oncologyen_US
dc.contributor.icrauthorVyse, Simonen
dc.contributor.icrauthorHuang, Paulen
dc.contributor.icrauthorNatrajan, Rachaelen


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