Whole-exome sequencing reveals the mutational spectrum of testicular germ cell tumours.
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Testicular germ cell tumours (TGCTs) are the most common cancer in young men. Here we perform whole-exome sequencing (WES) of 42 TGCTs to comprehensively study the cancer's mutational profile. The mutation rate is uniformly low in all of the tumours (mean 0.5 mutations per Mb) as compared with common cancers, consistent with the embryological origin of TGCT. In addition to expected copy number gain of chromosome 12p and mutation of KIT, we identify recurrent mutations in the tumour suppressor gene CDC27 (11.9%). Copy number analysis reveals recurring amplification of the spermatocyte development gene FSIP2 (15.3%) and a 0.4 Mb region at Xq28 (15.3%). Two treatment-refractory patients are shown to harbour XRCC2 mutations, a gene strongly implicated in defining cisplatin resistance. Our findings provide further insights into genes involved in the development and progression of TGCT.
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Chromosomes, Human, Pair 12
Neoplasms, Germ Cell and Embryonal
DNA Mutational Analysis
Polymorphism, Single Nucleotide
Genes, Tumor Suppressor
Proto-Oncogene Proteins c-kit
Molecular & Population Genetics
Sarcoma Molecular Pathology
Clinical Academic Radiotherapy (Huddart)
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Nature communications, 2015, 6 pp. 5973 - ?