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Ribosome. The complete structure of the 55S mammalian mitochondrial ribosome.

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Date
2015-04-02
ICR Author
Greber, Basil
Author
Greber, BJ
Bieri, P
Leibundgut, M
Leitner, A
Aebersold, R
Boehringer, D
Ban, N
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Type
Journal Article
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Abstract
Mammalian mitochondrial ribosomes (mitoribosomes) synthesize mitochondrially encoded membrane proteins that are critical for mitochondrial function. Here we present the complete atomic structure of the porcine 55S mitoribosome at 3.8 angstrom resolution by cryo-electron microscopy and chemical cross-linking/mass spectrometry. The structure of the 28S subunit in the complex was resolved at 3.6 angstrom resolution by focused alignment, which allowed building of a detailed atomic structure including all of its 15 mitoribosomal-specific proteins. The structure reveals the intersubunit contacts in the 55S mitoribosome, the molecular architecture of the mitoribosomal messenger RNA (mRNA) binding channel and its interaction with transfer RNAs, and provides insight into the highly specialized mechanism of mRNA recruitment to the 28S subunit. Furthermore, the structure contributes to a mechanistic understanding of aminoglycoside ototoxicity.
URI
https://repository.icr.ac.uk/handle/internal/4123
DOI
https://doi.org/10.1126/science.aaa3872
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Subject
Mitochondria
Animals
Swine
Humans
GTP-Binding Proteins
Aminoglycosides
Mitochondrial Proteins
Ribosomal Proteins
RNA, Messenger
RNA, Ribosomal, 16S
RNA, Transfer
Anti-Bacterial Agents
Binding Sites
Nucleic Acid Conformation
Protein Structure, Secondary
Mutation
Mitochondrial Membranes
Ribosome Subunits, Large
Language
eng
Date accepted
2015-03-06
License start date
2015-04-02
Citation
Science (New York, N.Y.), 2015, 348 (6232), pp. 303 - 308

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