Ribosome. The complete structure of the 55S mammalian mitochondrial ribosome.
Date
2015-04-17ICR Author
Author
Greber, BJ
Bieri, P
Leibundgut, M
Leitner, A
Aebersold, R
Boehringer, D
Ban, N
Type
Journal Article
Metadata
Show full item recordAbstract
Mammalian mitochondrial ribosomes (mitoribosomes) synthesize mitochondrially encoded membrane proteins that are critical for mitochondrial function. Here we present the complete atomic structure of the porcine 55S mitoribosome at 3.8 angstrom resolution by cryo-electron microscopy and chemical cross-linking/mass spectrometry. The structure of the 28S subunit in the complex was resolved at 3.6 angstrom resolution by focused alignment, which allowed building of a detailed atomic structure including all of its 15 mitoribosomal-specific proteins. The structure reveals the intersubunit contacts in the 55S mitoribosome, the molecular architecture of the mitoribosomal messenger RNA (mRNA) binding channel and its interaction with transfer RNAs, and provides insight into the highly specialized mechanism of mRNA recruitment to the 28S subunit. Furthermore, the structure contributes to a mechanistic understanding of aminoglycoside ototoxicity.
Collections
Subject
Mitochondria
Animals
Swine
Humans
GTP-Binding Proteins
Aminoglycosides
Mitochondrial Proteins
Ribosomal Proteins
RNA, Messenger
RNA, Ribosomal, 16S
RNA, Transfer
Anti-Bacterial Agents
Binding Sites
Nucleic Acid Conformation
Protein Structure, Secondary
Mutation
Mitochondrial Membranes
Ribosome Subunits, Large
Language
eng
Date accepted
2015-03-06
License start date
2015-04-02
Citation
Science (New York, N.Y.), 2015, 348 (6232), pp. 303 - 308
Publisher
AMER ASSOC ADVANCEMENT SCIENCE
Related items
Showing items related by title, author, creator and subject.
-
Insertion of the Biogenesis Factor Rei1 Probes the Ribosomal Tunnel during 60S Maturation.
Greber, BJ; Gerhardy, S; Leitner, A; Leibundgut, M; Salem, M; et al. (CELL PRESS, 2016-01-14)Eukaryotic ribosome biogenesis depends on several hundred assembly factors to produce functional 40S and 60S ribosomal subunits. The final phase of 60S subunit biogenesis is cytoplasmic maturation, which includes the ... -
Characterisation of the Phosphatidylinositol 3-Kinase Pathway in Non-Small Cell Lung Cancer Cells Isolated from Pleural Effusions.
Puglisi, M; Stewart, A; Thavasu, P; Frow, M; Carreira, S; et al. (KARGER, 2016-01-01)OBJECTIVES: We hypothesised that it was possible to quantify phosphorylation of important nodes in the phosphatidylinositol 3-kinase (PI3K) pathway in cancer cells isolated from pleural effusions of patients with non-small ... -
Microbiota- and Radiotherapy-Induced Gastrointestinal Side-Effects (MARS) Study: A Large Pilot Study of the Microbiome in Acute and Late-Radiation Enteropathy.
Reis Ferreira, M; Andreyev, HJN; Mohammed, K; Truelove, L; Gowan, SM; et al. (AMER ASSOC CANCER RESEARCH, 2019-11-01)PURPOSE: Radiotherapy is important in managing pelvic cancers. However, radiation enteropathy may occur and can be dose limiting. The gut microbiota may contribute to the pathogenesis of radiation enteropathy. We hypothesized ...